Three Electrodes Biosensor for Alcohol in Whole Blood Based on Multi-wall Carbon-nanotube
10.3724/SP.J.1096.2010.00389
- VernacularTitle:基于多壁碳纳米管的三电极血乙醇生物传感器的研究
- Author:
Shenghang ZHEN
;
Jun ZHENG
;
Chaoshi ZOU
;
Yan WANG
;
Yang ZHU
;
Shixiong DENG
;
Guoming XIE
;
Jian WANG
- Publication Type:Journal Article
- Keywords:
Blood alcohol;
Screen printing technique;
Multi-wall carbon nanotube;
Biosensor
- From:
Chinese Journal of Analytical Chemistry
2010;38(3):389-392
- CountryChina
- Language:Chinese
-
Abstract:
A novel disposable three electrodes blood alcohol biosensor strip was fabricated by a screen printing technique. Multi-wall carbon nanotube(MWCNT), Meldola′s(MB), alcohol dehydrogenase(ADH)and nicotinamide adenine dinucleotide cofactor (NAD+) were modified on the surface of the carbon working electrode. Then hydrophilic membrane was stuck in the outermost of the three electrodes to make a reaction camera of 5 μL. Experimental results indicated that the biosensor possessed good accuracy and stability, the linear response range was 0.5-20 mmol/L with correlation coefficient of 0.9949, detection limit was 0.22 mmol/L, and the response time was less than 15 s. Some influencing factors to the biosensor were investigated, such as the pH, temperature and interferences. Correlation analysis showed that there was a significant correlation between the methods of biosensor and the headspace vapor phase chromatography in 10 whole blood samples(r=0.97583). Small volume whole blood sucked using siphonage to detect blood alcohol directly and quantitatively was the obvious character of the biosensor.
