A role of p38 mitogen-activated protein kinase in regulation of NF-κB and MCP-1 expressions in HBZY-1 mesangial cell line
- VernacularTitle:p38丝裂原活化蛋白激酶对HBZY-1系膜细胞株表达NF-κB和MCP-1的调控
- Author:
Qianping WEI
;
Huacong DENG
;
Jie ZHAO
- Publication Type:Journal Article
- Keywords:
p38 mitogen-activated protein kinase;
NF-κB;
Monocyte chemoattractant protein-1;
Diabetic nephropathies;
Rat mesangial cell line HBZY-1
- From:
Chinese Journal of Endocrinology and Metabolism
2008;24(3):308-311
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship among p38 mitogen-activated protein kinase (p38MAPK), NF-KB and monocyte chemoattractant protein-1 (MCP-1), and to study the role of p38MAPK, NF-κB and MCP-1 in diabetic nephropathy. Methods Protein expressions of p38MAPK and NF-κB, and mRNA expression of MCP-1 were initially investigated in rat mesangial cell line HBZY-1, which were incubated separately with 25mmol/L glucose, 100nmol/L insulin, 100 μmol/L H2 O2 and 100mg/L advanced glycosylation end products (AGEs). The relationship among p38MAPK, NF-κB and MCP-1 expression Was observed by usingSB203580, a specific inhibitor of p38MAPK. Results The expressions of p38MAPK, NF-κB and MCP-1 were increased in HBZY-1 cells incubated separately with 25mmol/L glucose, 100nmol/L insulin, 100μmol/L H2 O2and 100 mg/L AGEs. Expressions of NF-κB and MCP-1 were significantly reduced when p38MAPK was inhibited by SB203580. Conclusion p38MAPK, NF-κB and MCP-1 are involved in development of diabetic nephropathy, and p38MAPK stimulation is essential for the expressions of NF-κB and MCP-1.
