Influence of danshen injection combined with dexamethasone on CD4+CD25+ regulatory T cells of asthmatic rats
- VernacularTitle:丹参注射液联合地塞米松对哮喘大鼠CD4+CD25+调节性T细胞的影响
- Author:
Keying XUE
;
Li CHENG
;
Chengguo WANG
;
Wei LI
;
Ming SHI
- Publication Type:Journal Article
- Keywords:
DANSHEN INJECTION/PD;
Dexamethasone/PD;
Antigens,CD4;
Receptors,interleukin-2;
T-lymphocytes
- From:
Journal of Chinese Physician
2008;10(6):746-748
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the immunological mechanism of inhibitory effect of Danshen injection combined with dexamethasone(DXM) on asthmatic airway inflammation.Methods 50 Wistar rats were randomly divided into normal control(NC),asthma,Danshen,DXM and Danshen+DXM group.Cytology study of Bronchoalveolar lavage fluid(BALF) was conducted.Pathology of lung tissue was done through HE.Flow eytometry was used to detect CD4+CD25+ regulatory T Cells(CD4+CD25+ Treg) ratio in peripheral blood mononuclear cells(PBMCs).IL-4 and IL-5 levels in BALF were detected by ELISA.Results Total cells number,percentage of lymphocytes,neutrophils and eosinophils(Eos) in BALF of the three treated groups were lower than that in asthma group(P<0.05,P<0.01),particularly in Danshen+DXM group,which showed significant difference as compared with the other two treated groups(P<0.05).There was severe inflammation in lung tissue of asthma group,moderate inflammation in Danshen group and DXM group,and no inflammation of Danshen+DXM group.CD4+CD25+ Treg/CD4+ T ratio in the three treated groups were higher than that in asthma group,and the levels of IL-4 and IL-5 were lower than those in asthma group(P<0.05).In Dansben+DXM group,it showed significant difference on the change of CD4+CD25+ Treg,IL-4 and IL-5 as compared with other treated groups(P<0.05).Conclision Danshen injection combined with DXM could suppress airway inflammation in asthmatic rats,which may be through increasing the expression of CD4+CD25+ Treg,decreasing the levels of IL-4 and IL-5 and resuming the balance of Th1/Th2.
