Rapid detection of five kinds of pathogens using single-tube multiplex PCR
- VernacularTitle:单管多重PCR技术快速检测五种性传播疾病的病原体研究
- Author:
Wenzhi TANG
;
Yuqiu ZHOU
;
Yongliang ZHANG
;
Wei LIU
;
Guifeng CAI
;
Xiaobo DAI
;
Xingrong TAN
- Publication Type:Journal Article
- Keywords:
Polymerase chain reaction/MT;
Neisseda gonorrhoens;
Mycophsma hominis;
Mycoplnsma genitulium;
Cldamydia trachomatis;
Ureaplasma urealyticum
- From:
Journal of Chinese Physician
2008;10(8):1024-1027
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a multiple PCR method that can be used to spontaneously detect five kinds ofpathogens such as NG,MH,MG,CT and UU.Method With the fluorescence-quantitative PCR technique in conjunction with another detection technique as the golden standard,evaluation was conducted on the sensitivity,specificity,accuracy and repeatability on the detection of 5 kinds of STD pathogens using single-tube multiplex PCR.Result The sensitivity,specificity and match rate of the method ale 10-9fg/μl,100%,97.8%respectively,and the repeatability of 5 continuous days of 20 clinical specimens is good.Conclusion Single-tube multiplex PCR technique provides a new method to detect 5 kinds of STD pathogens.
