P gene RT region mutations of hepatitis B virus in 74 patients with lamivudine-resistance
- VernacularTitle:74例经拉米夫定治疗的乙型肝炎病毒感染者耐药情况分析
- Author:
Zhongdong ZHANG
;
Huimin LIU
;
Tiefeng Lü
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Lamivudine;
Mutation;
Viral load
- From:
Chinese Journal of Clinical Infectious Diseases
2008;1(4):204-206
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate mutations at HBV P gene RT region in lamivudine-resistant patients. Methods Serum samples were collected from 74 hepatitis B patients resistant to lamivudine. HBV P gene RT region was amplified by PCR, and the PCR products were directly sequenced and the viral loads were detected. Results There were 7 forms of mutations in 74 patients harboring YMDD mutations detected in this study. The common mutation forms were rtM204V/rtL180M, rtM204I and rtM204I/rtL180M. The most frequent mutation positions were rtM204I (38.7%), rtM204V (21.8%), rtL180M (38.7%) and rtV173L (0.8%). Conclusions The mutation forms and positions of lamivudine-resistant viral strains are complex. Common mutation forms and positions are not directly correlated with the viral load, but some novel forms may be associated with it.
