Effect of insulin on the apoptosis of vascular endothelial cells induced by burn serum
10.3760/cma.j.issn.1671-0282.2009.01.015
- VernacularTitle:胰岛素对烧伤血清诱导血管内皮细胞凋亡的影响及机制
- Author:
Wanfu ZHANG
;
Dahai HU
;
Genfa LV
;
Yunchuan WANG
;
Xiongxiang ZHU
;
Feng GAO
- Publication Type:Journal Article
- Keywords:
Insulin;
Bum;
Endothelial cell;
Apoptosis;
Endothelial nitric oxide synthase(eNOS)
- From:
Chinese Journal of Emergency Medicine
2009;18(1):56-59
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effeets of insulin on the apoptosis of vascular endothelial cells cuinduced by burn$eruln in order to explore its possible mechamsm.Method Cultured human ECV304 cells were randomly divided into 33x)ups:control group,the ECV304 cells hured by 15%(V/V)rat normal,qertlm(t=6);bum semm group,the ECV3()4 cells simulated by 15%(V/V)self-made burn semm collected from rats with 30%TBSA full-thickness burns on the,back(n=6);and burn Serum+insulin group.the ECV304 cells cultared by insulin(10-7mol/L)and 15%(V/V) seIf-made rat bum serum(n=6).The transferase mediated nick end labeling(TUNEL) method was employed to measure the apoptosis of endothelial cells at 6 hours after stimulation.Meanwhile.immanohistochemical technique and Western blotting were used to determine the protein expressions of bcl-2 and eNOS.Data are expressed ills mean ±SEM.Statistical comparison was made using oneway analysis of vtriance.Significance was accepted at P<0.05.Results Compswith the control group,bum$erunl induced the apoptusis(18.5±3.1%)and down-regalated bcl·2(O.36±0.12)and p-eNOS(O.55±0.28)protein expressions of HUVECs(P<0.01).Burn 9AJ'unl+insulin significantly decreased the apoptosis(9.6 4-2.8%)and up-regulated bcl-2(0.944-0.25)and p-eNOS(0.89±0.16)protein expressions ofHU-VECs in comparison with the bum serllm group(P<0.01).eNOS showed no significant differences in three groups.Conclusions Insulin could markedly inhibit the apoptosis and up-regalate bcl-2 protein expression of HUVECs induced by bum serum,and its mec,harfism might involve the protein expression ofphosphorylated eNOS.
