Survivin as a factor of radioresistance to high-LET carbon ions in human hepatoma SMMC.7721 cells
10.3760/cma.j.issn.0254-5098.2009.01.001
- VernacularTitle:12C对人肝癌SMMC-7721细胞中survivin表达的影响
- Author:
Xiaodong JIN
;
Li GONG
;
Qiang LI
;
Jifang HAO
;
Ping LI
;
Qingfeng WU
;
Ling HE
;
Xinguo LIU
;
Zhongying DAI
- Publication Type:Journal Article
- Keywords:
SMMC-7721 cell;
Survivin;
Apoptosis;
RNA interference
- From:
Chinese Journal of Radiological Medicine and Protection
2009;29(1):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influences of survivin down-regulation on cell G2/M phase arrest,apeptosis and sensitivity to carbon ion irradiation. Methods Small interfering RNA (siRNA) targeting survivin mRNA was designed, in vitro chemo-synthesized and transfected into SMMC-7721 cells. Survivin mRNA expression in SMMC-7721 cells was measured by real-time PCR, and the apeptotic rates by Annexin-FTTC at 24 and 48 h after transfection. Cell G2/M phase arrest after transfection was assessed with flow eytometry as well. Cellular sensitivity to high-LET carbon ions was determined by means of colony-forming assay. Results The expressions of survivin at mRNA level were down-regulated to be 59% and 39% in relation to the non-treated cells at 24 and 48 h after siRNA transfeetion, respectively. G2/M phase arrest in SMMC-7721 cells at 24 h after transfection was observed while much more obvious at 48 h. The apeptotic rate of SMMC-7721 cells was 21.41 % at48 h after survivin siRNA transfection, which was significantly higher than that of the cells transfected with negative siRNA. Moreover, a decreased clonogenic survival in siRNA treated group was shown. Conclusion Down-regulation of survivin gene expression in SMMC-7721 cells by siRNA could effectively induce cell apeptosis and G2/M phase arrest, and enhance the cellular radiosensitivity to high-LET heavy ions.
