Development of an Improved Animal Model of Overactive Bladder: Transperineal Ligation versus Transperitoneal Ligation in Male Rats.
10.5534/wjmh.2016.34.2.137
- Author:
Woo Hyun KIM
1
;
Woong Jin BAE
;
Jung Woo PARK
;
Jin Bong CHOI
;
Su Jin KIM
;
Hyuk Jin CHO
;
U Syn HA
;
Sung Hoo HONG
;
Ji Youl LEE
;
Sung Yeoun HWANG
;
Sae Woong KIM
Author Information
1. Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Korea. ksw1227@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Rats;
Urinary bladder, overactive;
Urinary bladder neck obstruction
- MeSH:
Animals*;
Humans;
Interleukin-8;
Interleukins;
Ligation*;
Male*;
Models, Animal*;
Necrosis;
Oxidative Stress;
Rats*;
Superoxide Dismutase;
Urinary Bladder;
Urinary Bladder Neck Obstruction;
Urinary Bladder, Overactive*
- From:The World Journal of Men's Health
2016;34(2):137-144
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: We compared a transperineal ligation model and a transperitoneal ligation model in male rats to determine which animal model of overactive bladder (OAB) was more useful based on cystometrography, estimations of oxidative stress, and measurements of pro-inflammatory cytokine levels. MATERIALS AND METHODS: Male rats were randomly divided into three groups (n=15 in each): the control group, the transperineal ligation group, and the transperitoneal ligation group. Four weeks after the ligation procedure, cystometrography was performed and oxidative stress, pro-inflammatory cytokine levels, and histologic changes were evaluated. Oxidative stress was assessed by measuring 8-hydroxy-20-deoxyguanosine and superoxide dismutase, and pro-inflammatory cytokine activity was investigated by measuring levels of interleukin (IL)-6, IL-8, and tumor necrosis factor-α. RESULTS: The transperineal model led to results similar to those observed for the transperitoneal model, namely (1) increased voiding frequency and reductions in the non-voiding contraction interval and the maximal vesical pressure, (2) increased levels of oxidative stress markers, (3) increased pro-inflammatory cytokine levels, and (4) fibrotic changes in the bladder tissue. CONCLUSIONS: We suggest that the transperineal procedure can be used as an alternative OAB model in male rats.
