Quantitative analysis of cell-free fetal DNA levels in maternal plasma with DOWN syndrome and normal pregnancies
10.3760/cma.j.issn.1008-6315.2009.07.005
- VernacularTitle:孕DOWN综合征胎儿妇女血浆游离胎儿DNA浓度定量研究
- Author:
Jianmin SUN
;
Yuedi TAN
- Publication Type:Journal Article
- Keywords:
Cell-free fetal DNA;
DOWN syndrome;
Real-time quantitative PCR
- From:
Clinical Medicine of China
2009;25(7):684-686
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the application of real-time quantitative PCR in quantification of cell-free fetal DNA maternal plasma in patients bearing fetuses affected with DOWN syndrome. Methods Cell-free fetal DNA in maternal serum was isolated from 30 samples(7 male DOWN syndrome fetal ,3 female DOWN syndrome fe-tal,14 male euploid fetal,6 female euploid fetal). Cell-free fetal DNA levels in maternal serum were measured using real-time quantitative PCR using SRY as marker. Results The median cell-free fetal DNA levels in pregnant carry-ing male fetuses(n=7) and the controls (pregnant carrying male euploid fetuses,n=14)were 318.03±96.74 ge-nome-equivalents/ml and 154.40±39.43 genome-equivalents/ml of maternal serum,respectively (t=3.33,P=0.004 ),which was o in women with female fetuses. Conclusion The cell-free fetal DNA levels in pregnant women with DOWN syndrome fetuses are higher than that in pregnant women with normal fetuses.
