Transcript levels and functional analysis of DNA methyltransferases in CD4+ T cells of MRL/lpr mice

VernacularTitle:DNA甲基化转移酶在MRL/lpr狼疮鼠CD4+T淋巴细胞中的表达水平研究及功能分析
Author: Min YUAN ; Lijuan MU ; Hanjuan CUI ; Xiaobing LUO ; Xinfang HUANG ; Yijun ZHENG ; Yuanjia TANG ; Nan SHEN
Publication Type:Journal Article
Keywords: Lupus erythematosus,systemic; DNA methylation; Mice,inbred MRL lpr,CD4positive T-lymphocytes
From: Chinese Journal of Rheumatology 2009;13(8):521-524
CountryChina
Language:Chinese
Abstract: Objective To investigate the expression levels of DNA methyltransferases 1, 3A and 3B in CD4+ T cells of MRL/lpr mice, and explore their relationship with the expression levels of methylation-sensitive genes (ITGAL, CD70). Methods CD4+ T ceils were isolated from spleens of 16-week-old MRL/lpr and BALB/c control mice by anti-CIM antibody labeled magnetic beads. Transcription levels of DNA methyhransferases 1, 3A and 3B and methylation-sensitive genes(ITGAL, CD70) were measured by real-time mice when compared with BALB/c control mice and the difference was significant (P<0.05), while the expression of DNMTI and DNMT3A showed a tendency of decrease (P>0.05). The mRNA expression of CD70 was significantly higher (P<0.01), but the expression of ITGAL had no significant difference between the two P<0.01 ). Conclusion Decreased expression of DNMT3B may attribute to the elevated expression of methylation-seusitive gene CD70, thus lead to the dysfunction of CD4+ T cell and play an important role in the pathogenesis of SLE.