Experimental study the cardiomyogenic differentiation of bone marrow mesenchymal stem cell induced by 5-azacytidine in a "Cardiac-like" milieu
10.3760/cma.j.issn.1008-6315.2009.09.019
- VernacularTitle:"心肌样"环境下5-氮胞苷诱导骨髓间充质干细胞分化的实验研究
- Author:
Xiang LING
;
Jichun LIU
;
Bentong YU
;
Qicai WU
- Publication Type:Journal Article
- Keywords:
Bone marrow mesenchymal stem cell;
5-azacytidine Cardiomyocyte;
Cardiac-like milieu
- From:
Clinical Medicine of China
2009;25(9):950-952
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effectivity of 5-azacytidine(5-Aza) inducing the cardiomyogenic SCs were isolated and purified from Sprague-Dawley (SD) rats. Some BMSCs were induced with 5-Aza,group Ⅰ was not induced with 5-Aza and the third generation of cells were used for the next experiment; group Ⅱ were cultured and induced with 5-Aza( 10 μmol/L) at the third day of the culture for 24 h; group Ⅲ were induced with 5-Aza( 10 μmol/L) for 24 h when the third generation of cell were fused; group Ⅳ were induced with 5-Aza( 10 μmol/L) for 24h at the third day of primary cuhure,which were induced with 5-Aza( 10 μmol/L) for 24 h before the third gener-phosis was found in the BMSCs among groups. CD34 was negatively expressed and CD29 and CD44 were positively gle 5-Aza inducing group(group Ⅲ ) was much higher than that in no 5-Aza inducing group( group Ⅰ ) [ MHC : (6. 82±2.05 ) % vs (3.61±1.14) % ], cTnI: (5.63±1.86) % vs (2.76±0.82) %, P < 0.05 ], but was significantly lower than the percentage in the double 5-Aza inducing group ( group Ⅳ ) [ MHC : ( 12.18±3.16) % vs ( 6.82±2. 05)% ] ,cTni:(9.93±2.79)% vs(5.63±1.86)% ,P<0.05]. Conclusions 5-Aza could promote the cardio-myogenic differentiation of BMSCs in a "Cardiac-like" Milieu. 5-Aza double induing is better than the single indu-cing.
