Co-circulation of Two Genotypes of Hepatitis A Virus from Sporadic Cases in Northeastern Area of Seoul, Korea.
10.3343/kjlm.2008.28.5.371
- Author:
Soo Jin YOO
1
;
Dong Dae SEO
;
Won Choong CHOI
;
Oh Joong KWON
;
Jae Chan PARK
;
Bo Moon SHIN
Author Information
1. Department of Laboratory Medicine, Sanggye Paik Hospital, Inje University College of Medicine, Seoul, Korea. bmshin@unitel.co.kr
- Publication Type:Original Article ; English Abstract
- Keywords:
Hepatitis A virus;
Acute hepatitis;
Genotype
- MeSH:
Adolescent;
Adult;
Amino Acid Sequence;
Child;
Female;
Genotype;
Hepatitis A/*virology;
Hepatitis A Virus, Human/classification/*genetics/isolation & purification;
Humans;
Immunoglobulin M/blood;
Korea;
Male;
Middle Aged;
Molecular Sequence Data;
Phylogeny;
RNA, Viral/analysis;
Reverse Transcriptase Polymerase Chain Reaction;
Sequence Analysis, DNA;
Viral Structural Proteins/genetics;
Young Adult
- From:The Korean Journal of Laboratory Medicine
2008;28(5):371-377
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: In previous studies, most hepatitis A virus (HAV) isolates had been genotype IA in Korea. Recently, a small number of different genotypes were reported with an upsurge of acute hepatitis by HAV. We investigated the distribution of HAV genotypes. METHODS: RNA was extracted from anti-HAV IgM positive sera which were collected from March 2007 to February 2008 at a tertiary care hospital in Northeastern Seoul, Korea. Nested reverse transcription (RT)-PCR and direct sequencing for VP1/P2A region of the HAV were performed. RESULTS: A total of 699 cases with suspected acute hepatitis were tested for anti-HAV IgM, and positive results were obtained in 56 sera (8.0%), which were collected 2 to 15 days (median, 7 days)after the onset of symptoms. Of the 56 seropositive samples, 52 (92.9%) were positive for HAV RNA, among which 28 isolates (53.8%) belonged to genotype IA and the remaining 24 (46.2%) belonged to genotype IIIA. Both IA and IIIA genotypes were isolated from 6-7 neighboring administrative districts throughout the year without geographic or seasonal restrictions. CONCLUSIONS: Co-circulation of two distinct HAV genotypes (IA and IIIA) was observed from the northeastern Seoul for the year studied.
