Detection of T cell receptor gamma gene rearrangements in mycosis fumgoides
10.3760/cma.j.issn.0412-4030.2009.12.010
- VernacularTitle:蕈样肉芽肿T细胞受体γ单克隆性基因重排研究
- Author:
Yiping MA
;
Xianmei LU
;
Guizhi ZHOU
;
Xuechao CHEN
- Publication Type:Journal Article
- Keywords:
Mycosis ftmgoides;
Gene rearrangement,gamma-chain T-cell antigen receptor
- From:
Chinese Journal of Dermatology
2009;42(12):828-830
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the diagnostic significance of T cell receptor gamma gene rearrangemerits in mycosis fungoides (MF), so as to develop a sensitive diagnosis tool. Methods A total of 50 specimens were collected, including 33 skin lesion specimens and 2 lymph specimens from 30 patients with MF,15 skin lesion specimens from 15 patients with inflammatory dermatoses. PCR was performed with specific primers targeting TCR V gamma 8, 9, 10, 11 to detect T cell receptor gamma gene rearrangement. Results Monoclonai rearrangements of TCR gene was observed in 88% (29/33) of specimens from patients with MF and 33% (5/15) of samples from patients with inflammatory dermatoses. Conclusions The detection of TCR gene rearrangements, as an ancillary test, is useful in the diagnosis and differential diagnosis of MF.
