Cerebral ischemia induces astrocytes to express granulocyte colony stimulating factor and glial cell fine-derived neurotrophic factor
10.3760/cma.j.issn.1006-7876.2009.10.012
- VernacularTitle:脑缺血诱发星形胶质细胞粒细胞集落刺激因子受体和胶质细胞源性神经营养因子的表达
- Author:
Langfeng SHI
;
Chuanzhen Lü
;
Baoguo XIAO
- Publication Type:Journal Article
- Keywords:
Brain ischemia;
Astrocytes;
Receptors,granulocyte colony-stimulating factor;
Glial cell line-derived neurotrophic factor
- From:
Chinese Journal of Neurology
2009;42(10):694-698
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the neuroprotective mechanisms of granulocyte colony stimulating factor (G-CSF) in ischemic brain injury. Methods Brain tissues were taken out from MCAO and sham operated Sprague-Dawley rats 7 days after operation. The expression of G-CSFR, GDNF, MAP2 and GFAP was measured by using immunofluorescence co-staining. Results The expression of G-CSFR and GDNF were widely distributed in the neurons in normal brain tissues, not in the astrocytes. However, in ischemic peripheral zone, part of G-CSFR and GDNF positive cells merged with GFAP positive cells, in normal brain tissues, most G-CSFR positive cells were co-expressed with GDNF. Conclusion Cerebral ischemia induces astrocytes to express G-CSFR and GDNF, suggesting that endogenous neuroprotection by cerebral ischemia may be related with the expression and production of G-CSFR and GDNF in astrocytes in ischemic peripheral zone.
