An experimental study on role of hIL-10-MSCs in heterologous T cell proliferation

VernacularTitle:hIL-10基因修饰骨髓间充质干细胞对异种T淋巴细胞增殖的影响及机制研究
Author: Jian NIU ; Bin LIU ; Yewei ZHANG ; Xiangnong LI
Publication Type:Journal Article
Keywords: Mesenchymal stem cell; IL-10; T-lymphocytes cell; Immune tolerance
From: Chinese Journal of Hepatobiliary Surgery 2010;16(2):138-141
CountryChina
Language:Chinese
Abstract: Objective To observe the effects of hIL-10-MSCs on heterologous T cell prolifera-tion. Methods hlL-10 was cloned by RT-PCR and then hIL-10/pLOX-cwGFPs was construct. The lentivirus was transfected into cavy MSCs and cell culture supernatant was tested for IL-10 protein by ELISA assay. The effects of hIL-10-MSCs on heterologous T cell proliferation were determined by CCK-8. The effect of peripheral blood mononuclearcell secretion IFN-γ was tested by EIASA. Results hIL-10/pLOX-cwGFP were constructed successfully and hIL-10-MSCs cell culture supernatant of IL-10 protein were increased obviously, hIL-10-MSCs could inhibit heterologous T cell proliferation sig-nificantly(P<0.05) but could not induce T cell proliferation (P<0.05). Adding IL-2 could reverse this inhibition(P<0.05), hIL-10-MSCs cell culture supernatant could inhibit peripheral blood mono-nuclearcell secretion IFN-γ. Conclusion hIL-10-MSCs may play an important role in significant im-munosuppression of heterologous T cell proliferation and suppression of secretion IFN-γ by peripheral blood mononuclear cells.