Studies on mechanism of treatment of granulocyte colony-stimulating factor,recombinant human interleukin-11 and recombinant human interleukin-2 on hematopoietic injuries induced by 4.5 Gy γ-rays irradiation in beagles
10.3760/cma.j.issn.0254-5098.2010.03.002
- VernacularTitle:细胞因子治疗4.5 Gyγ射线照射比格犬造血损伤的机制初探
- Author:
Ming LI
;
Hongling OU
;
Shuang XING
;
Haixiao HUANG
;
Guolin XIONG
;
Ling XIE
;
Yanfang ZHAO
;
Zhenhu ZHAO
;
Ning WANG
;
Jinxiang WANG
;
Jingcheng MIAO
;
Nankang ZHU
;
Qingliang LUO
;
Yuwen CONG
;
Xueguang ZHANG
- Publication Type:Journal Article
- Keywords:
Acute radiation sickness;
Cytokines;
Hematopoietic injuries;
CD34+cells;
Cell cycle;
Apoptosis;
Beagles
- From:
Chinese Journal of Radiological Medicine and Protection
2010;30(3):239-242
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of treatment of granulocyte colony-stimulating factor(rhG-CSF),recombinant human interleukin-11(rhIL-11)and recombinant human interleukin-2 (rhIL-2)on hematopoietic injuries induced by 4.5 Gy60 Coγ-ray irradiation in beagles,and to provide experimental evidence for the clinical treatment of extremely severe myeloid acute radiation sickness (ARS).Methods Sixteen beagle dogs were given 4.5 Gy60 Co γ-ray total body irradiation(TBI),then randomly assigned into irradiation control group,supportive care group or cytokines+supportive care (abbreviated as cytokines)group.In addition to supportive care,rhG-CSF,rhlL-11 and rhIL-2 were administered subcutaneously to treat dogs in cytokines group.The percentage of CD34+cells,cell cycle and apoptosis of nucleated cells in peripheral blood were examined by Flow cytometry.Results After 4.5 Gy 60 Co γ-ray irradiation,the CD34+cells in peripheral blood declined obviously(61.3%and 52.1% of baseline for irradiation control and supportive care group separately).The cell proportion of nucleated cells in Go/G1 phase was increased notably(99.27% and 99.49% respectively).The rate of apoptosis(26.93% and 21.29% separately)and necrosis(3.27% and 4.14%,respectively)of nucleated cells were elevated significantly when compared with values before irradiation(P<0.05) 1 d post irradiation.When beagles were treated with cytokines and supportive care,the CD34+cells in peripheral blood were markedly increased(135.6% of baseline).The effect of G0/G1 phase blockage of nucleated cells became more serious(99.71%).The rate of apoptosis(5.66%)and necrosis(1.60%)of nucleated cells were significantly lower than that of irradiation control and supportive care groups 1 d after exposure.Conclusions Cytokines maybe mobilize CD34+cells in bone marrow to peripheral blood,indce cell cycle block at G0/G1 phase and reduce apoptosis,and eventually cure hematopoieticinjuries induced by irradiation.
