Effects of Ang-( 1-7 ) on the apoptosis of cultured endothelial cells induced by Ang Ⅱ
10.3760/cma.j.issn.1008-1372.2010.06.012
- VernacularTitle:血管紧张素-(1-7)对血管紧张素Ⅱ诱导人脐静脉内皮细胞凋亡的影响
- Author:
Huiyu YANG
;
Zhiming YANG
;
Yunfei BIAN
;
Maolian LI
;
Nana ZHANG
;
Fen GAO
;
Chuanshi XIAO
- Publication Type:Journal Article
- Keywords:
Angiotensin Ⅰ/PD;
Angiotensin Ⅱ/PD;
Umbilical veins/CY;
Endothelium,vascular/CY;
Apoptosis/DE
- From:
Journal of Chinese Physician
2010;12(6):748-751
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Ang-(1-7) on the apoptosis in human umbilical vein endothelial cells (HUVECs) induced by Ang Ⅱ.Methods HUVECs were isolated and cultured.Cultured HUVECs were incubated for 24 h with Ang-(1-7), Ang Ⅱ, Ang-(1-7) A-779, Ang-(1-7) + Ang Ⅱ, A-779 + Ang Ⅱ + Ang-( 1-7), respectively.Cultured HUVECs without incubating stimulator were chosen as controls.The apoptosis of endothelial cells were detected by flow cytometry.Results The apoptosis of endothelial cells in HUVECs were upregulated by AngⅡ ( 10-6 mol/L) (25.60% ±3.17% vs 2.32% ±0.24%, P ＜0.005).Compared with the AngⅡ group, Ang-(1-7) dose-dependently inhibited the apoptosis of endothelial cells in HUVECs ( (20.04% ± 2.21% ,16.04% ± 1.32 %, 10.04% ±2.05,7.79% ±1.50% vs AngⅡ group 25.60% ±3.17%, P ＜0.05 , P ＜0.05).The effects of Ang-(1-7) could be blocked by A-779 (23.37% ±0.75% vs 20.04% ± 2.21%, 16.04% ± 1.32,10.04% ± 2.05% ,7.79%± 1.50%, P ＜ 0.05 ).Conclusion Ang-(1-7) can attenuate the apoptosis of endothelial cells induced by Ang Ⅱ in HUVECs in a dose-dependent manner.The effects of Ang-(1-7) could be blocked by A-779( P＜0.05).
