Experimental study of IRAK-4 expression in hypoxic neurons of rats
10.3760/cma.j.issn.1001-8050.2010.07.023
- VernacularTitle:缺氧大鼠神经元中IRAK-4的表达
- Author:
Jiangtao LI
;
Shengli HU
;
Jun HU
;
Peng DU
;
Hua FENG
;
Yunfeng YANG
;
Linjie WEI
;
Gang ZHU
- Publication Type:Journal Article
- Keywords:
Anoxia;
Interleukin-1 receptor associated kinase-4;
Neurons;
Secondary brain injury
- From:
Chinese Journal of Trauma
2010;26(7):648-652
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the dynamic changes of interleukin-1 receptor-associated kinases (IRAK-4) in hypoxic neurons and explore their role in regulation of inflammatory reaction. Methods The B35 cells exposed to hypoxia of 3% O2,5% CO2 and 92% N2 were cultured for 1,3,6, 12,24,48,72 and 96 hours respectively. Then, mRNA and protein expressions of IRAK-4 were detected by RT-PCR and Western blot, the expression of IRAK-4 in the cells were observed by laser scanning con-focal microscope (LSCM), and the concentration of IL-6 was measured by ELISA method. Results After hypoxia, the mRNA and protein expressions of IRAK-4 were increased at one hour, reached the peak at six hours (P<0.05), kept at a high level at 12 hours (P<0.05) , but decreased gradually to the normal oxygen level at 24 hours (P < 0.05) and to below the normal oxygen level at 48 hours (P < 0.05). Immunofluorescence results showed that the fluorescence intensity of IRAK-4 was gradually increased with time. The changes of IL-6 in the supernatant were positively correlated with protein expression of IRAK-4 (r =0.84, P < 0.05 ). Conclusions Hypoxia can increase the expression of IRAK-4 at transcription and translation levels in a certain period of time, which may participate in down-stream inflammatory reaction and lead to increase of IL-6 expression.
