Purification of Lectin (Abrus precatorius) and its application in detecting pathogenic bacteria
- Author:
Nguyen Hanh Phuc
- Publication Type:Journal Article
- Keywords:
Lectin
- MeSH:
Lectins;
Abrus;
Diagnosis;
Bacteria
- From:Journal of Preventive Medicine
2005;15(5):112-116
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Lectin (APA) was purified from jequirity bean (Abrus precatorius) by affinity chromatography on insoluble ovomucoid. Lectin (APA) and conjugate Lectin-biotin have been used in ELLA technique (Enzyme Linked Lectinosorbent Assay) to detect some pathogenic bacteria. Based on its specific bioavailability to the poD-Gal sugar compounds in cell membrane, lectin (APA) was capable of differentiating B. anthracis and B. cereus from S. flexneri, S. sonnei, C. diphteria, and Salmonella and Staphylococcus aureus.
