Effect of microRNA-let-7a on apoptosis in melanoma cell line A375

VernacularTitle:微小RNA-let-7a对黑素瘤细胞系A375细胞凋亡的影响
Author: Zhenying WANG ; Jie ZHANG ; Yan WANG ; Yi LIU ; Yiqun JIANG ; Xuesi ZENG ; Jianfang SUN
Publication Type:Journal Article
Keywords: Melanoma; Apoptosis; Caspase 3; Cell line,tumor; microRNA-let-7a
From: Chinese Journal of Dermatology 2010;43(8):575-578
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of microRNA-let-7a on apoptosis in melanoma cell line A375 and its mechanism. Methods The expression of microRNA-let-7a was detected in A375 cells and melanocytes by real-time PCR. Then, microRNA-let-7a mimics was transfected into A375 cells followed by the measurement of apoptosis and caspase-3 protein expression by flow cytometry and Western blotting, respectively. Results The expression level of microRNA-let-7a was reduced by 0.462 folds in A375 cells compared with melanocytes. The apoptosis rate was 47.4% in A375 cells transfected with microRNA-let-7a mimics, significantly higher than that in untransfected A375 cells (16.9%). A significant decline was observed in the expression of caspase-3 protein in A375 cells after transfection. Conclusion microRNA-let-7a can promote the apoptosis and downregulate caspase-3 protein expression, in A375 human malignant melanoma cells.