Apoptostic effect of arsenic trioxide on human coronary smooth muscle cells in vitro
10.3760/cma.j.issn.1671-0282.2010.08.004
- VernacularTitle:三氧化二砷对人冠状动脉平滑肌细胞促凋亡作用的体外实验
- Author:
Tianzhu LUAN
;
Hongliang JI
;
Songbin FU
;
Lijun ZHOU
;
Weimin LI
;
Yonglin HUANG
- Publication Type:Journal Article
- Keywords:
Arsenic trioxide;
Smooth muscle cell;
Apoptosis;
Coronary angioplasty;
Restenosis
- From:
Chinese Journal of Emergency Medicine
2010;19(8):797-801
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of arsenic trioxide on human coronary smooth muscle cells(HCSMCs). Method After the subculture of HCASMCs, cells were divided into 4 groups: control group and three arsenic trioxide (4.0 μmol/L) groups as the arsenic agent co-cultured with cells for 12,24 and 48 h. Flow cytometry and TUNEL were used for counting the ntmnber of cell apoptosis. RT-PCR was used to detect the expression of E2F-1 mRNA and Western blot was used to measure the levels of Bcl-2 ond Bax. Results Arsenic trioxide inhibited the proliferation of HCASMCs. When arsenic trioxide was 4.0 μmol/L, the living cells were ( 8.44 ±0.10) × 105/mL,signifiantly than that of control group (16.44 ± 1.34) × 105/mL, P ＜ 0.05. This inhibition of proliferation cycle was produced by affecting S and G2-M phase, which did not appear in the control group. Apoptotic cells increased from 16.0% ± 3.1% to 38.7% ± 2.7% (P ＜ 0.05) detected by TUNEL. The arsenic agnet decreased the Bcl-2 level and increased Bax. The expression of E2F-1 mRNA was decreased in 4.0 μmol/L arsenic trioxide group. Conclusions Arsenic trioxide exerts the apoptotic effect on human coronary smooth muscle cells.
