Expression of human papillomavirus DNA in keratoacanthoma tissue
10.3760/cma.j.issn.0412-4030.2010.09.005
- VernacularTitle:人乳头瘤病毒DNA在角化棘皮瘤中的表达
- Author:
Xuechao CHEN
;
Xianmei LU
;
Guizhi ZHOU
- Publication Type:Journal Article
- Keywords:
Keratoacanthoma;
Papillomavirus,human;
In situ hybridization
- From:
Chinese Journal of Dermatology
2010;43(9):614-616
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the infection rate of human papillomavirus (HPV) types in keratoacanthoma (KA) tissue, and to study the distribution of HPV DNA-positive cells. Methods In situ hybridization was performed to detect HPV subtypes 6/11, 16/18, and 31/33 in paraffin-embeded tissue samples from 46 patients with KA and 34 nomal human controls. Results Mucosal HPV DNA was positive in 31 (67.39%)of the 46 KA samples, and mixed infection rate of HPV amounted to 83.87% (26/31) in these positive samples.The DNA of tested mucosal HPV types existed mainly in episomal status. Positive reactions were observed within or at the edge of nuclei. HPV infected cells showed a stripe-, slice-like or focal distribution mainly in superficial middle lamina of spinous layer at the bottom or lateral margin of crater-shaped epidermal depression. No mucosal HPV DNA was noted in normal control samples. Conclusions Compared with normal controls, patients with KA are infected with mucosal HPV at a higher frequency, and mucosal HPV DNA mainly exists in episomal status in KA tissue, hinting that HPV infection play a certain role in the pathogenesis of KA.
