Carboxy-methyl-chitosan inhibits interleukin-1β-induced NF-kappa B activation in rat chondrocytes

VernacularTitle:羧甲基壳聚糖抑制白细胞介素-1β诱导的大鼠软骨细胞核因子-κB活化
Author: Wanjun DING ; Shiqing LIU ; Hao PENG ; Yaming LI
Publication Type:Journal Article
Keywords: Chitosan; Chondrocytes; NF-kappa B; Interleukin-1; Nitric oxide
From: Chinese Journal of Rheumatology 2010;14(9):602-605
CountryChina
Language:Chinese
Abstract: Objective To investigate the efffect of carboxy-methyl-chitosan (CM-chitosan) on NF-kB activation and iNOS expression in IL-1β-induced rat chondrocytes, and the mechanism is explored. The statistical treatment was ANOVA. Methods The chondrocytes derived from knee joint of rats were cultured in vitro. 10 ng/ml IL-1β with or without CM-chitosan of varied concentrations (100, 200, 300 μg/ml) was added into the culture medium. Inducible NO synthase (iNOS) was examined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. The expression of NF-κBn and NFκBc as well as I-κB were examined by Western blotting. Results CM-chitosan could significantly decrease iNOS expression of IL-1β-induced chondrocytes. Degradation of I-κB and NF-κB nuclear translocation were reversed by carbox-ymethyl-chitosan. Conclusion The study has demonstrated that CM-chitosan can inhibit the synthesis of inflammatory mediators in rat chondrocytes when stimulated with IL-1β through a NF-κB-dependent mechanism.