The effects of unilateral urethral obstruction on the expression of AQPs and its implications

VernacularTitle:单侧输尿管梗阻对肾脏水通道蛋白表达的影响及临床意义
Author: Youkong LI ; Xianjue ZHANG ; Guanghua YANG ; Jiajie ZHOU ; Kun DING ; Jianguo WANG ; Min ZHU ; Zhongjun CHEN ; Jinmin ZENG ; Yixiang LIAO
Publication Type:Journal Article
Keywords: Ureteral obstruction/ME; Kidney/ME; Aquaporin 1/ME; Aquaporin 2/ME; URINE △/PH
From: Journal of Chinese Physician 2010;12(10):1332-1336
CountryChina
Language:Chinese
Abstract: Objective To investigate the change of AQP1 and AQP2 before and after the release of obstruction and explore the relationship between reabsorption dysfunction of renal tubule and the change of AQPs. Methods The model of unilateral ureter obstruction (UUO) was established by surgery. Western blot and immunohistochemistry were used to study the expression of AQPs before and after obstruction. Results In UUO model, both AQPs began to down-regulate one day after obstruction, the expression of both AQPs became lower one day after the release of obstruction. And they started to up-regulate 7 day after the release of obstruction. AQP2 became normal since 14 days after the release of obstruction, and AQP1 became normal since 21 days after the release of obstruction. Conclusion The expression of AQP1 and AQP2 were descended in hydronephrosis. The dysfunction of renal tubule and the osmotic-dependent polyuria after the release of obstruction in UUO were caused by the down - regulation of AQPs.