Co-stimulatory signal GL50-ICOS expression in thyroid of Graves' disease and its immune pathogenetic significance
10.3760/cma.j.issn.1000-6699.2010.11.012
- VernacularTitle:Graves病甲状腺共刺激分子GL50-ICOS的表达及其免疫病理意义
- Author:
Lei CHEN
;
Ting LI
;
Honger YIN
;
Songguang JU
;
Gehua YU
;
Lian JIANG
;
Fengming WANG
;
Ming WU
;
Xueguang ZHANG
- Publication Type:Journal Article
- Keywords:
Costimulatory molecule;
Graves' disease;
Thyroid
- From:
Chinese Journal of Endocrinology and Metabolism
2010;26(11):967-970
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of co-stimulatory molecules, GL50-ICOS, in thyroid tissue of patients with Graves' disease (GD) and to explore their relationship with the immune pathogenesis of GD.Methods RT-PCR, Western blot, immunohistochemistry were applied to detect the expression of GL50-ICOS in thyroid of GD. Thyrocytes were cultured in the absence or presence of pro-inflammatory cytokines. The expression of GL50 on thyroid follicular cells (TFC) was further measured by flow cytometry. Results (1) In GD patients,the percentage of CD4+ CD28- T cells was significantly increased as compared with the control healthy individuals. The expression of co-stimulatory molecule ICOS was up-regulated. (2) The mRNA level of ICOS was significantly increased in GD patients than that in nontoxic goiter(NTG) patients(P＜0.01). (3)Compared with NTG control group, the GL50 protein expression was much higher in thyroid tissues of GD patients (P ＜0.01). (4)The results of immunohistochemistry showed that GL50 expression was observed in all GD thyroid tissues, while no expression of GL50 was detected in NTG thyroid tissues(P＜0. 01). (5) The expression of GL50on primary cultured thyroid follicular cells was significantly increased under the stimulatation of pro-inflammatory cytokines in vitro. Conclusion GL50-ICOS is expressed abnormally in thyroid tissue of patients with GD.
