The clinical significance of peripheral blood hematopoietic stem/progenitor cell in patients with rheumatoid arthritis
10.3760/cma.j.issn.1007-7480.2010.12.003
- VernacularTitle:类风湿关节炎患者外周血CD34+造血干/祖细胞的检测及其意义
- Author:
Jie CHEN
;
Long QIAN
;
Mingming QIN
;
Guosheng WANG
;
Xiangpei LI
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Antigens,CD34;
Haematopoietic stem cells
- From:
Chinese Journal of Rheumatology
2010;14(12):808-810
- CountryChina
- Language:Chinese
-
Abstract:
Objective To measure the number of peripheral blood CD34+ hematopoietic stem/progenitor cells (HSC/HPC) expression of CD34 in the peripheral blood of patients with rheumatoid arthritis and exploreits relationship with clinical manifestations. Methods CD34+ HSC/HPCs in the peripheral blood of RA patients (n=32) and healthy controls (n=16) were detected using flow cytometry. The relationship between the frequency of HSC/HPCs, mean fluorescence intensities (MFI) of CD34 and clinical manifestations and rheumatoid factor (RF), anti-cyclic citrullinated peptide (CCP) antibodies, disease activity score (DAS) 28,X rays stages and healthy assessment questionnaire (HAQ) were analyzed. Student's t-test and pearont test were used for statistical analysis. Results Frequency of CD34+ HSC/HPC in the peripheral blood of RA patients was decreased compared with normal controls [ (0.13±0.09)% vs (0.38±0.21)%, P＜0.05 ], CD34 MFIwere higher in RA patients than those in the normal controls (57±33 vs 3111, P＜0.05). The frequency was positively correlated with the number of (RBC red blood cell), (Hb hemoglobin), and was negatively correlated with C-reactive protein (CRP), and the MFI of RA patients was positively correlated with healthy assessment questionnaire (HAQ) and X ray stages, but negatively correlated with the number of platelets.Conclusion CD34+ HSC/HPC of the peripheral blood of RA patients are significantly abnormal, which is characterized by decreased CD34+ hematopoietic stem cell, and the decrease is positively correlated with RBC and Hb, but negatively correlated with CRP. CD34+ hematopoietic stem cell may play an important role in the pathogenesis of RA.
