Inhibition of invasiveness of pancreatic carcinoma cell line PANC-1 by suppression of MMP-2 gene expression using RNA interference
10.3760/cma.j.issn.1007-8118.2010.11.021
- VernacularTitle:靶向MMP-2的RNAi抑制胰腺癌PANC-1细胞侵袭性的研究
- Author:
Xuefeng ZHU
;
Dechun LI
;
Yijun CHEN
;
Jianwei XU
;
Jili GU
;
Dongming ZHU
;
Jiannong CEN
- Publication Type:Journal Article
- Keywords:
Pancreatic carcinoma;
Matrix metalloproteinase-2;
RNA interference;
Invasiveness
- From:
Chinese Journal of Hepatobiliary Surgery
2010;16(11):863-866
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effects of RNA interference on expression of matrix metalloproteinase-2(MMP-2)gene and invasiveness of human pancreatic cancer cell line PANC-1 in vitro.Methods Small interference RNA targeting MMP-2 gene was designed and constructed to plasmid pGCsi-U6.Recombinant plasmids were transfected to pancreatic carcinoma PANC1 cells with Lipofectamine 2000.The efficiency of transfection was evaluated by flow cytometry.RQPCR was used to detect the expression of MMP-2 mRNA.The expression of MMP-2 protein was determined by ELISA.The invasiveness of PANC-1 cells was measured by transwell chamber experiment.MTT assay was used to detect the proliferation and growth of PANC-1 cells.Results Sequencing confirmed that the MMP-2 siRNA plasmid was successfully constructed.The best efficiency of transfecting recombinant plasmid was 82.1%.After transfection of the MMP-2 siRNA plasmid, the MMP-2 gene expression of PANC-1 cells was suppressed to 71.74 %(P＜0.05),and protein expression of MMP-2 fell to 49.82%(P＜0.05).The corresponding inhibition ratio of invasiveness was 33.0%(P＜0.05).There was no marked difference in proliferation rate measured by MTT assay among different groups(P＞0.05).Conclusions RNAi targeting MMP-2 can suppress invasiveness of PANC-1 cells in vitro.This suggests that MMP-2 could be a target for gene therapy of pancreatic carcinoma.RNAi is expected to open up a new prospect for tumor therapy.
