The study on the role of transcription factor GATA binding protein 3 in familial Alzheimer's disease pathogenesis
10.3760/cma.j.issn.1006-7876.2011.03.003
- VernacularTitle:转录因子GATA结合蛋白3在家族性阿尔茨海默病发病机制中的作用
- Author:
Wei QIN
;
Aihong ZHOU
;
Xiumei ZUO
;
Fen WANG
;
Zhe CHENG
;
Jianping JIA
- Publication Type:Journal Article
- Keywords:
Alzheimer disease;
Presenilin-1;
Insulin degrading enzyme;
GATA3 transcription factor
- From:
Chinese Journal of Neurology
2011;44(3):159-162
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanisms of decreasing insulin degrading enzyme (IDE) level by mutation V97L in the gene presenilin 1 (PS-1).Methods Transcription factor GATA binding protein 3 (GATA-3) activity was assessed by protein/DNA array and verified by Western blot in SH-SY5Y cells transfected by PS-1 mutation V97L.Results Protein/DNA array and Western blot revealed that there was increased transcript factor activity (5.5 times high) and protein level of GATA-3 in V97L-PS-1 transfected SH-SY5Y cells.Transcription factor GATA-3 can bind to the IDE promoter and negatively control the IDE transcription level.Conclusion PS-1 mutation V97L may regulate the transcription of IDE via GATA-3, and subsequently involve in deposition of Aβ42 and development of Alzheimer's disease.
