Comparison of human cell IFN-β production induced by bluetongue virus dsRNA and polyinosinic polycytidylic acid
- VernacularTitle:异源蓝舌病毒dsRNA与PolyI:C诱导人源体细胞产生IFN-β的比较研究
- Author:
Ying DAI
;
Donge CHEN
;
Jing GAO
;
Jun HU
;
Changyuan DONG
- Publication Type:Journal Article
- Keywords:
Bluetongue virus;
BTV dsRNA;
Polyinosinic-polycytidylic acid;
Interferon
- From:
Chinese Journal of Microbiology and Immunology
2008;28(3):217-220
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the capability of bluetongue virus(BTV)dsRNA inducing IFN-β from human cells.Methods Artificial complex interfemn inducer polyinosinic-polycytidylic acid (PolyI:C).BTV and BTV dsRNA were added to A549(human lung cancer cell)and HEL(human lung normal cells)culture system in difierent concentrations.IFN-β in culture median was detected by ELISA.Results Though all of the 3 reagents could induce IFN-β,BTV dsRNA significanay induced the highest level of IFN-β.The production of IFN-β was induced by BTV dsRNA in dose dependence.BTV dsRNA induced IFN-β level from HEL Was higher than that from A549(P<0.05).Conclusion BTV dsRNA Can induce IFN-β from human cells effectively,which shows its potential of an endogenous IFN-β inducer.
