MicroRNA expression profiling in hydroxycamptothecin-resistant human colon cancer cell line by microarray
- VernacularTitle:应用芯片技术筛查人结肠癌羟基喜树碱多药耐药相关微RNA的研究
- Author:
Jinlu TONG
;
Zhihua RAN
;
Xiang CHEN
;
Xitao XU
;
Fang NIE
;
Shudong XIAO
- Publication Type:Journal Article
- Keywords:
Colonic neoplasms;
MicroRNAs;
Drug resistance,multiple;
Gene expression microarry analysis
- From:
Chinese Journal of Digestion
2008;28(4):246-249
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of a novel regulatory molecule-microRNA in the hydroxycamptothecin-resistant human colon cancer cell line SW1116/HCPT in order to provide a new reversal target for muhidrug resistance.Methods MicroRNA expression profiling in the hydroxycamptothecin-resistant human colon cancer cell line SW1116/HCPT were detected by microRNA array using microRCURYTM LNA Array V8.1 to screen multi-drug resistance(MDR)-related microRNAs.Specific stem-loop primers were used for reverse-transcribing cDNA and the expression of some MDR-related microRNAs were analyzed by the real-time PCR.Results The absorbance ratios of total RNA used for total RNA preparation was further confirmed by denaturing agarose gel electrophoresis.Compared to SW1116,28 microRNAs were down-regulated and 36 microRNAs were up-regulated in SW1116/HCPT cell line.The expression of two down-regulated microRNAs(hsa-miR-452 and hsa-miR-373*)and one up-regulated microRNA(hsa-miR-506)were confirmed by real-time PCR.The results of hsa-miR-452 and hsa-miR-506 were consistent with microRNA array nalysis,however,the expression of hsa-miR-373* may play a key role in the process of hydroxycamptothecin-resistant human colon cancer cell line SW1116/HCPT.
