Study on gene polymorphism of HIV-1 B' nef and its influence on disease progression in northern China
10.3760/cma.j.issn.0254-5101.2010.05.014
- VernacularTitle:中国HIV-1 B'亚型毒株nef基因多态性及其对疾病进展影响的研究
- Author:
Xihua DONG
;
Xiaoxu HAN
;
Di DAI
;
Bin ZHAO
;
Xiaoli ZHANG
;
Hong SHANG
- Publication Type:Journal Article
- Keywords:
HIV-1;
nef;
Gene polymorphism;
Long-term nonprogress
- From:
Chinese Journal of Microbiology and Immunology
2010;30(5):449-455
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the polymorphism of nef gene and conservation level of functionally important domains of nef as well as their influences on HIV-1 disease progression of HIV-1 B'infected individuals in northern China.Methods 30 long term nonprogressors(LTNPs)and 42 typical progressors (TPs)were selected.Provirus DNA was extracted from whole blood sample.The full nef gene was amplified by nested-PCR.PCR product was sequenced directly after purification.Phylogenetic analysis and amino acid sequence mutation was applied on nef sequences to explore the differences between LTNPs and TPs.Results At position 15,the S15R/K/N substitution was detected.The frequency of TPs(64.29%)wsa higher than LTNPs(33.33%,P<0.01,0R=3.60);R21K/E/H/I.Q,TPs and LTNPs mutation frequency was 59.52%and 93.33%(P<0.005,OR=0.11);At position 39,K39R/E/N was only detected in TPs(23.81%,P<0.005).Conclusion No significant deletion or defect associated with disease progression wsa detected in nef gene of HIV-1 B'.But it suggested that K/E/H/I/Q mutation at 21 st amino acid of nef associated the disease nonprogression.R/K/N at 15 th amino acid of nef and R/E/N mutation at 39th amino acid of nef associated the disease progression in.HIV-1 B'.All domaias of nef amino acids sequences were comparatively conservative.
