The impact of Pan3CSK4 on the platelet activation and the expression of TLR2 on it
10.3760/cma.j.issn.0254-5101.2010.06.006
- VernacularTitle:Pam3CSK4对血小板活化及其TLR2表达的影响
- Author:
Benquan WU
;
Mingjun ZHI
;
Hui LIU
;
Yuqi ZHOU
;
Tiantuo ZHANG
- Publication Type:Journal Article
- Keywords:
Platelet;
Toll-like receptor 2;
Platelet aggregation;
Platelet activation;
CD62p
- From:
Chinese Journal of Microbiology and Immunology
2010;30(6):516-520
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investgate role of TLR2 in the activation, the innate immune and inflammation of human platelets. Methods Human washed platelets were separated from healthy people(n=5) and were stimulated with different concentrations(1μg/ml, 5μg/ml, 10μg/ml) of TLR2 agonistPam3CSK4(a synthetic bacterial lipoproteins). Then the platelet aggregation rate, the expression of CD62p and TLR2 on the platelet surface were measured. Results The platelet aggregation rate were (28.32±5.67)%, (52.56±8.54)% and (76.24±11.23)%, respectively, at concentration of 1μg/ml, 5μg/mland 10μg/ml of Pam3CSK4, more than (12.83±2.43)% at 0μg/ml of it. In addition, the expression of CD62p were (18.45±2.66)%, (22.45±2.04)%, (29.53±4.08)%, respectively at above concentration of Pam3 CSK4, more than (11.20±1.67)% of CD62p at control group(P<0.01). The expression of TLR2 was not significantly increased at a lower concentration of Pam3CSK4(1μg/ml) with (16.85±6.10)% compared with(10.81±3.99)% at the control group. However, it were (21.15±9.90)% and (22.52±9.26)%, respectively, at a higher concentration(5μg/ml, 10μg/ml)more than(10.81±3.99)% at the control group(P<0.05). Conclusion Pam3CSK4 induce aggregation, activation and the up-regulation of TLR2 of platelet by stimulating TLR2 receptor of it. Thereafter, TLR2 play an important role in the innate immune of platelet.
