Indirect ELISA with Recombinant GP5 for Detecting Antibodies to Porcine Reproductive and Respiratory Syndrome Virus
10.1007/s12250-011-3154-9
- Author:
Yan CHEN
;
Hong TIAN
;
Jianhui HE
;
Jinyin WU
;
Youjun SHANG
;
Xiangtao LIU
- Publication Type:Journal Article
- Keywords:
Porcine reproductive and respiratory syndromevirus(PRRSV);
GP5 protein;
Indirect ELISA
- From:
Virologica Sinica
2011;26(1):61-66
- CountryChina
- Language:Chinese
-
Abstract:
Porcine reproductive and respiratory syndrome is caused by the PRRS virus(PRRSV), which has six structural proteins(GP2, GP3, GP4, GP5, M and N). GP5 and N protein are important targets for serological detection by enzyme-linked immunosorbent assay(ELISA)and other methods. Toward this goal, we developed an indirect ELISA with recombinant GP5 antigens and this method was validated by comparison to the LSI PRRSV-Ab ELISA kit. The results indicated that the optimal concentration of coated recombinant antigen was 0.2 μg/well for a serum dilution of 1:40. The rate of agreement with the LSI PRRSV-Ab kit was 88.7%(266/300). These results support the potential use of recombinant GP5 as an antigen for indirect ELISA to detect PRRSV antibodies in pigs.
