Study of qepA among clinical isolates of Klebsialla pneumoniae

VernacularTitle:肺炎克雷伯菌的喹诺酮外排基因qepA的检测及菌株同源性分析
Author: Xiaobo MA ; Lin LIN ; Xiuyu SONG ; Lili FANG ; Jiaqin ZHANG ; Gangsen ZHENG ; Yanqing ZHENG
Publication Type:Journal Article
Keywords: Plasmid-mediated; qepA; K. pneumoniae
From: Chinese Journal of Microbiology and Immunology 2011;31(1):47-50
CountryChina
Language:Chinese
Abstract: Objective To investigate the prevalence of qepA, quinolone efflux protein, among 41 unique clinical strains of K. pneumoniae producing ESBLs and to study the qepA-bearing isolates using the Diversilab system. Methods Identification and antimicrobial susceptibility were performed by Vitek-2 Compact System. Screening of qepA was carried out by PCR amplification. The NCBI BLAST program was utilized for sequence comparisons. qnr-bearing strains was evaluated by the Repetitive-sequence-based PCR(Rep-PCR) employing the Diversilab system. And the existence of rmtB was detected among these qepA contained isolates. Results qepA were detected in 5 isolates( 12.2% ). The Rep-PCR profiles produced by the Diversilab system showed that 2/5 of isolates were indistinguishable. And 60% of qepA-positive isolates were detected to harbor rmtB gene. Conclusion The data suggest the emergence of qepA-borne K. pneumoniae.