Effect of AP-2α gene transfection on proliferation and invasion potential of human colon cancer SW480 cells in vitro
- VernacularTitle:AP-2α基因转染对人类结肠癌SW480细胞体外增生及侵袭能力的影响
- Author:
Ying WANG
;
Xiaoqin LAN
;
Meining LI
;
Yuehong ZHANG
;
Xiaobo LIANG
;
Niuliang CHENG
- Publication Type:Journal Article
- Keywords:
Colon neoplasms;
Transcription factor AP-2;
Gene transfection;
SW480 cells;
Cell proliferation;
Invasiveness
- From:
Cancer Research and Clinic
2008;20(6):376-379
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the proliferation and invasion potential of human colon cancer SW480 cells transfected by AP-2α gene in vitro. Methods pcDNA3.1 (+)-AP-2α was created by cloning AP-2α eDNA into the EcoRI site of poDNA3.1 (+). LipofectamineTM 2000 Reagent was used to mediate the transfection of pcDNA3.1(+)-AP-2α and pcDNA3.1 (+), and normal SW480 celia were cultured as a negative control. The mRNA and protein level of AP-2α in the cells of each group were detected 48 h after being transfected with plasmids above by RT-PCR and Western blotting analysis. The cell proliferation and invasion potential were examined by colony formation assay and Transwell invasion assay respectively. Results Lack of AP-2α protein expression in SW480 cells was verified by western blot analysis. After being transfected with AP-2α gene, the mRNA amount and protein expression increased dramatically, while the colony formation efficiency decreased(P <0.05), the cell proliferation in soft agar was inhibited, and the ability of its invasion dropped off(P <0.05) in vitro. Conclusion AP-2α gene suppresses the proliferation and invasion potential of human colon cancer SW480 cells in vitro.
