Experimental study of the effect of RNAi to silence STAT3 gene expression on the growth of T24 and 5637 bladder cancer cells
- VernacularTitle:RNAi沉默STAT3基因表达对膀胱癌细胞T24及5637生长影响的实验研究
- Author:
Wenming MA
;
Jinying GUO
;
Yan CHEN
;
Yangan WEN
;
Zhiguang TU
- Publication Type:Journal Article
- Keywords:
Urinary bladder neoplasms;
RNA interference;
STAT3 transcription factor
- From:
Cancer Research and Clinic
2008;20(6):372-375
- CountryChina
- Language:Chinese
-
Abstract:
Objective Signal transducers and activators of transcription 3 (STAT3) silenced by RNA interference (RNAi) technique were used to induce the apoptosis and growth inhibition in T24 and 5637 bladder cancer cells. Methods Three recombinant plasmids pGenesil-1-shRNA-STAT3 was constructed and transfected into T24 and 5637 cells. The expression of STAT3 gene was detected by RT-PCR and Western blotting. FCM was used to observe the apoptosis in T24 and 5637 cells. Results pGenesil-1-shRNA-STAT3 was successfully constructed, and transfected into T24 and 5637 cells. RT-PCR and Western blot analysis demonstrated that pGenesil-1-shRNA-STAT3 could significantly inhibit the expression of STAT3 in T24 and 5637 cells; FCM results show that it could suppress the growth of 1'24 and 5637 cells. Conclusion pGeneSiI-1-shRNA-STAT3 could significantly inhibit STAT3 expression, suppress the growth of T24 and 5637 cells.
