Infectivity of different human immunodeficiency virus strains for mucosal epithelial cell lines
- VernacularTitle:人免疫缺陷病毒对不同黏膜上皮细胞系的感染能力的研究
- Author:
Yue LI
;
Hui ZHAO
;
Jun DU
;
Yu QUAN
;
Hui XING
;
Qimin CHEN
;
Yiming SHAO
;
Guibo YANG
- Publication Type:Journal Article
- Keywords:
Human immunadeficiency virus (HIV-1);
Mucosal epithelial cells;
Infection;
Inte-gration
- From:
Chinese Journal of Microbiology and Immunology
2008;28(7):577-581
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the infectivity between laboratory adapted human inununodefi- ciency virus(HIV-1) and primary HIV-1 isolates for different mucosal epithelial cell lines. Methods Mu-cosal epithelial cells Caco-2, T-84, HeLa and lymphocyte MT-4 were infected with laboratory adapted HIV-1 SF33 and 2 primary HIV-1 isolates (02010561, 02010141). Culture supernatant and cells were collected respectively on 3-4 days interval after virus inoculation. The former was tested for HIV-1 antigen P24 level and viral load, and the latter was tested for total viral DNA and integrated viral DNA. Results All 3 virus strains could infect MT-4 cells and integrate into their genome. Only HIV-1 SF33 could infect Caco-2 cells but could not integrate into their genomic DNA. Both HIV-1 SF33 and 02010561 infected HeLa cells but only integration of HIV-1 SF33 was detected. All the 3 HIV-1 strains infected T-84 cells but only the integra-tion of HIV-1 SF33 and 02010141 was observed. Conclusion Although laboratory adapted and primary HIV-1 strains are able to infect human mucosal epithelial cell lines, transient or productive infection estab-lished in different mucosal epithelial cells is dependent on the character of cells and virus strains.
