Phosphoproteomic analysis of differential expression of AGS cellular proteins in response to Helicobanter pyiori infection
10.3760/cma.j.issn.0254-5101.2009.05.016
- VernacularTitle:幽门螺杆菌感染后人胃腺癌上皮细胞微量磷酸化蛋白质差异分析
- Author:
Di XIAO
;
Yanyan SONG
;
Fei ZHAO
;
Lihua HE
;
Fanliang MENG
;
Huifang ZHANG
;
Jianzhong ZHANG
- Publication Type:Journal Article
- Keywords:
Affinity adsorption;
Phosphorylated proteins;
2-DE;
Pathogenic mechanism
- From:
Chinese Journal of Microbiology and Immunology
2009;29(5):449-453
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the differential expression of trace phosphorylated proteins in human gastric adenocarcinoma epithelial (AGS) cells infected by Helicobacter pylori. Methods H. pylori 26695 strain infected AGS cells 4 h and AGS cells was cultivated for 4 h as a comparison. The proteins of AGS and comparison AGS cells were extracted. Their phosphorylated proteins were enriched by metal ion af-finity adsorption enrichment techniques. After desalinated and purified the phosphorylated proteins samples were separated by 2-dimensional polyacrylamide gel electrophoresis (2-DE) technique. Computer assisted image analysis was used to analyze the differential proteomic expression. The significantly differentially ex-pressed proteins were unambiguously assigned identities by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/TOF). Results Fifteen kinds of proteins were down-regulated, 4 kinds of new proteins were observed, 1 kind of proteins were up-regulated, 1 kind of proteins unexpression. The 21 proteins that were significantly differentially expressed , including cellular calcium ion homeostasis, transcription, interpretation, protein folding and transport, ribosomal assembly, centrosome replication, chromosome stability, cellular structure, cellular proliferation and apoptosis. Conclusion H. priori can cause a wide range change to human gastric adenocarcinoma epithelial cell protein pheshorylation. This change character has great significance to further comprehensive understanding of the pathogenesis of H. pylori.
