Comparison of the application of ARMS-PCR and RFLP-PCR techniques in research of single nucleotide polymorphisms about MMP-2 in cervical cancer

VernacularTitle:扩增受阻突变体系-PCR和限制性酶切片段多态性-PCR技术在研究子宫颈癌MMP-2单核苷酸多态性中的比较
Author: Weifeng LIN ; Suhui WU ; Haixia SHANG ; Shiqian TANG ; Li ZHANG
Publication Type:Journal Article
Keywords: Uterine cervical neoplasms; Polymerase chain reaction; Polymorphism; singlenucleotide; MMP-2
From: Cancer Research and Clinic 2009;21(11):742-744
CountryChina
Language:Chinese
Abstract: Objective To compare the advantages and disadvantages of the amplification refractory mutation system -polymerase chain reaction (ARMS-PCR) with restriction fragment length polymorphism polymerase chain reaction(RFLP-PCR) technology, then explore its application in detection and analysis of single nucleotide polymorphism (SNP). Methods By using ARMS-PCR and RFLP-PCR the polymorphisms of the MMP-2 in cervical cancer and the normal-control group were analyzed. The research sites were-1306 C/T.The final result was verified by DNA sequencing. Results The results of amplified used ARMS-PCR showed that the genotypes of the SNP site in MMP-2 gene promoter were CC, CT, TT, and the DNA fragments were corresponding with the results of DNA sequencing. Conclusion ARMS-PCR technology has the advantages of lower cost time-saving simple operation and reliable results, while has a high value for research of SNP.