Treatment of metanephric mesenchymal cells transplantation for adriamycin-induced chronic nephropathy rats
10.3760/cma.j.issn.1001-7097.2009.12.O11
- VernacularTitle:后肾间充质细胞移植治疗大鼠慢性阿霉素肾病
- Author:
Yuqing JIAO
;
Zhuwen YI
;
Xiaojie HE
;
Xihong LIU
;
Qingnan HE
;
Danlin HUANG
;
Xiqiang DANG
;
Xiaochuan WU
;
Yan CAO
;
Shuanghong MO
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cell transplantation;
Fibrosis;
Matrix metalloproteinaes;
Chronic kidney disease;
Adriamycin
- From:
Chinese Journal of Nephrology
2009;25(12):930-935
- CountryChina
- Language:Chinese
-
Abstract:
Objecfive To detect the functional repair of metanephric mesenchymal cells (MMCs) transplantation in adriamycin (ADR)-induced glomerulopathy rats. Methods A total of 90 Sprague-Dawley female rats were randomly divided into three groups:ADR group (n=40,rats were injected via the tail vein with O.25 mg ADR/100 g body weight on days 1 and 21),ADR- MMCs group(n=40,rats were injected via the tail vein with 5×10~6-7×10~6 MMCs 8 weeks after the second ADR administration),control(n=10).All the rats were scarified 8 weeks after MMCsinjection.Pathology and collagen IV expression in renal tissue were examined.Moreover,matrix metalloproteinases 2 (MMP-2) and matrix metallopmteinases 9 (MMP-9) expression in the renal tissue were also detected with immunohistochemistry,and quantity analysis of protein and gene was further demonstrated with Westem blot and RT-PCR analysis,respectively. Results There were no significant differences in tubulointerstitial injury score and glomerulosclerosis degree between ADR group and ADR-MMCs group(P>0.05).Compared with ADR group,collagen Ⅳ and MMP-2 expression decreased, MMP-9 expression incrased in renal tissue of ADR-MMCs group, and the difference was significant (P<0.05). Conclusion MMCs transplantation may have potentially therapeutic effect on renal tissue fibrosis of adriamyein-induced glomerulopathy in rats, and the signaling pathways of MMPs appear to be involved in these processes.
