Application of DNA chip techniques for Yq microdeletion analysis in infertile males.
- Author:
Suman LEE
1
;
Hyun Suk JOO
;
Sook Hwan LEE
;
Jung Eun PARK
;
Jong Man KIM
;
Jae Hoon HWANG
;
Key Seung CHO
;
Seung Yong HWANG
Author Information
1. Genome Research Center for Reproductive Medicine and Infertility, Infertility Medical Center, CHA General Hospital, College of Medicine, Pochon CHA University, Seoul 135-913, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
DNA chip;
male infertility;
microdeletion;
molecular diagnosis;
STS
- MeSH:
Chromosome Deletion;
Chromosomes, Human, Y/*genetics;
DNA Mutational Analysis/methods;
Electrophoresis, Agar Gel;
Female;
Humans;
Infertility, Male/*diagnosis/*genetics;
Male;
Oligonucleotide Array Sequence Analysis/*methods;
Polymerase Chain Reaction;
Predictive Value of Tests;
Research Support, Non-U.S. Gov't;
Seminal Plasma Proteins/*genetics;
Sensitivity and Specificity;
Sequence Tagged Sites;
*Sex Chromosome Aberrations
- From:Experimental & Molecular Medicine
2004;36(2):179-184
- CountryRepublic of Korea
- Language:English
-
Abstract:
Our aim was to apply DNA chip technology as a diagnostic tool in infertility research and clinics. Six loci, including a sex-determining region on the Y chromosome and five sequence-tagged sites in azoospermia-factor regions were investigated in infertile male patients. Our method produced a sensitive signal, which showed the presence or absence of the STS regions on the Y chromosome. The results from 93 patients with non- obstructive azoospermia, oligoathenoteratozoospermia, or oligozoospermia were identical when analyzed with either the DNA chip technique or conventional PCR-gel electrophoresis. We have demonstrated its application in the molecular diagnosis of male infertility. This system provides an economic and high-throughput method for detecting the deletion of genomic DNA sequences of large groups of infertile patients, and a completely new approach to male infertility screening. The application of DNA chip technology to identify Yq deletions can also facilitate our understanding of male infertility.
