Basophil Activation Test for Kampo Medicines :Proper Concentration to Avoid False Positive Result
10.3937/kampomed.67.67
- VernacularTitle:漢方薬に対する好塩基球活性化試験
- Author:
Naoki MANTANI
;
Hiroshi OKA
;
Ayao SUZUKI
;
Motoko AYABE
;
Mayumi SUZUKI
;
Hiroshi KAMIYAMA
- Publication Type:Journal Article
- Keywords:
Kampo medicine;
basophil activation test;
concentration;
false positive
- From:Kampo Medicine
2016;67(1):67-71
- CountryJapan
- Language:Japanese
-
Abstract:
Recently, basophil activation test (BAT) has been applied to the diagnosis of drug allergy. We performed BAT for various Kampo medicines taken by 12 cooperators to evaluate the concentration which arouse nonspecific reaction during incubation in BAT. When whole blood of each was incubated for 24 hours with each Kampo medicine, false positive results were frequently observed. After 1-hour incubation with Kampo medicine at high concentration (1/312.5), false positive results were sometimes observed. These results suggest that in the diagnosis of Kampo-medicine adverse reactions, BAT should be performed in the condition of 1-hour incubation with lower concentration (1/1250 or lower).
