Effect of Okara Reacted to Subcritical Water on Antitumor and Antipromoter Activity
10.1625/jcam.9.129
- VernacularTitle:亜臨界水処理後のオカラにおける抗腫瘍性及び抗プロモーター作用
- Author:
Masato NISHI
;
Toshiki ENOMOTO
;
Ryousuke SAKAI
- Publication Type:Journal Article
- Keywords:
Okara;
antitumor;
antipromoter;
sub-critical water
- From:Japanese Journal of Complementary and Alternative Medicine
2012;9(2):129-135
- CountryJapan
- Language:Japanese
-
Abstract:
Objective: The purpose of this study was to confirm the effect of Okara-puree (okara after the sub-critical water reaction) on antitumor and antipromoter activity.
Method: The antipromoter activity was investigated by colony method using JB6 Cl 41 cells and 3-3 MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) method. We examined the antitumor effect by measuring the survival days and the bodyweight of the mice in which colon cancer cells had been transplanted in the soles of the left foot.
Result: Okara-puree DMSO extract inhibited EGF(epidermal growth factor)-induced colonization of JB6 Cl 41 cells. In the mice transplanted with cancer cells, more survivors were seen in the Okara-puree group compared to control during the period day 10 to day 20. Furthermore,the mice in the Okara-puree group showed a longer survival time compared to control even after day 22.
Conclusion: Unlike unprocessed okara, Okara-puree shows promise for development as a functional food material with antitumor and antipromoter effects.
