Anti-human Cytomegalovirus Activity of Hot Water Extract from Sasa senanensis Rehder
10.1625/jcam.6.17
- VernacularTitle:クマザサ熱水抽出物によるヒトサイトメガロウイルスの抑制効果の解析
- Author:
Rie YAMADA
;
Kazuhiko AKUZAWA
;
Changxiao BI
;
Zhuan LI
;
Hidetaka SADANARI
;
Keiko MATSUBARA
;
Tsugiya MURAYAMA
- Publication Type:Journal Article
- Keywords:
human cytomegalovirus;
Sasa senanensis antiviral activity;
ganciclovir
- From:Japanese Journal of Complementary and Alternative Medicine
2009;6(1):17-25
- CountryJapan
- Language:Japanese
-
Abstract:
Objective: Anti-Human cytomegalovirus (HCMV) activity of Kumazasa (Sasa senanensis Rehder) extract were investigated in the human embryonic lung (HEL) fibroblast cell.
Method: This study examined the effect of hot water extract from Sasa senanensis Rehder on HCMV replication, viral-specific major immediate early (IE) gene expression, and protein synthesis.
Results: Treatment of HCMV-infected HEL cells with that Sasa senanensis Rehder extract inhibited the cytopathic effect and viral production in a dose-dependent manner. Further, real-time RT-PCR assay and Western blotting analysis revealed that Sasa senanensis Rehder extract decreased expression of the HCMV IE gene in virus-infected HEL cell.
Conclusion: These results suggest that Sasa senanensis Rehder extract have anti-HCMV activity by inhibitting of IE gene expression.
