Functional Analyses of Oligonol by Novel Neutrophil Activity Measurement System
10.1625/jcam.10.59
- VernacularTitle:新規好中球機能検査法によるオリゴノールの機能解析
- Author:
Katsuhiko SUZUKI
;
Miki TOMARI
;
Kaoru SUGAMA
;
Kentaro KITADATE
;
Shigeki MIURA
;
Hiroshi YOSHIOKA
;
Yuichi MORI
- Publication Type:Journal Article
- Keywords:
polyphenol;
oligonol;
antioxidant activity
- From:Japanese Journal of Complementary and Alternative Medicine
2013;10(1):59-62
- CountryJapan
- Language:Japanese
-
Abstract:
We examined the effects of polyphenols on migratory activity of neutrophils into the hydrogel and their production of reactive oxygen species (ROS). Origonol and lychee polyphenol were diluted to medium in wide-range concentrations. Each solution was added on hydrogel, and the mixture of blood and luminol was layered on it in each tube. The ROS production was measured by luminol-dependent chemiluminescence, whereas the cell count in the hydrogel was quantified as migratory activity of neutrophils. The migratory activity of neutrophils was not affected, whereas ROS production of neutrophils was significantly decreased above 100 μg/ml, but increased below the concentration.
