Study on Anti-Proliferative Activities of Cultured Cordyceps Militaris on Cancer Cells
10.1625/jcam.10.51
- VernacularTitle:サナギタケ培養液のがん細胞増殖抑制作用に関する研究
- Author:
Msayuki HIGASHINO
;
Hiroshi HORIE
;
Masakatu ITO
;
Keiko TANAKA
;
Yasuki FUKUDA
;
Shozo NISHIDA
;
Kazuya MURATA
;
Hideaki MATSUDA
- Publication Type:Journal Article
- Keywords:
Cordyceps Militaris anti-proliferative activity of cancer cell lines;
Uracil;
alkali metal salts
- From:Japanese Journal of Complementary and Alternative Medicine
2013;10(1):51-57
- CountryJapan
- Language:Japanese
-
Abstract:
Cordyceps militaris has been known to produce an anticancer agent, cordycepin. Investigation on optimum culture condition for C. militaris had been performed. In the research program for discovering a novel function in the culture of C. militaris, the culture media was applied to a proliferation assays using various cell lines. The media showed significant anti-proliferative activities against al cell lines, especially to human leukemia cell line HL-60. The activity-guided purification of active ingredient was performed to obtain uracil. To the best of our knowledge, uracil has not been reported to possess anti-proliferative activity. However, the uracil obtained from the culture media was subjected to ICP-MS analysis to reveal that sodium, potassium and magnesium were found to co-exist with uracil, which might show anti-proliferative activity. Further study on the mechanism of the expression of the activity is now underway.
