Antiapoptotic Effect of the Leukemia Associated Gene MLAA-34 in HeLa Cells.
- Author:
Peng-Yu ZHANG
1
,
2
;
Xuan ZHAO
3
;
Wen-Juan ZHANG
4
;
Wang-Gang ZHANG
5
;
Yin-Xia CHEN
5
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, Neoplasm; genetics; metabolism; Apoptosis; Apoptosis Regulatory Proteins; genetics; metabolism; Arsenicals; Cell Cycle; Cell Proliferation; HeLa Cells; Humans; Lentivirus; Oxides; Transfection
- From: Journal of Experimental Hematology 2016;24(2):363-368
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the antiapoptotic effect of leukemia-associated gene MLAA-34 in HeLa cells.
METHODSThe MLAA-34 recombinant lentiviral expression vector was constructed, and the stably transfected HeLa cell line with high expression of MLAA-34 was set up; As(2)O(3) was used to induce apoptosis; the MTT assay, colony formation test and flow cytometry were used to detect the ability of cell proliferation, colong formation, apoptosis and cell cycle changes respectively.
RESULTSAfter treatment with As(2)O(3), the survival rate of HeLa cells with MLAA-34 overexpression was significantly higher than that of the control cells, and the colony formation ability of MLAA-34 significantly increased, and the high expression of MLAA-34 gene significantly decreased the apoptosis rate of HeLa cells, and decreased the proportion of G(2)/M phase cells.
CONCLUSIONThe leukemia-associated gene MLAA-34 has been comfirmed to show antiapoptotic effect in HeLa cells which are induced by As(2)O(3).
