Induction of nucleoside phosphorylase in Enterobacter aerogenes and enzymatic synthesis of adenine arabinoside.
- Author:
Xiao-Kun WEI
1
,
2
;
Qing-Bao DING
;
Lu ZHANG
;
Yong-Li GUO
;
Lin OU
;
Chang-Lu WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cytidine; pharmacology; Cytidine Monophosphate; pharmacology; Enterobacter aerogenes; enzymology; Enzyme Induction; Pentosyltransferases; biosynthesis; Vidarabine; biosynthesis
- From: Journal of Zhejiang University. Science. B 2008;9(7):520-526
- CountryChina
- Language:English
- Abstract: Nucleoside phosphorylases (NPases) were found to be induced in Enterobacter aerogenes DGO-04, and cytidine and cytidine 5'-monophosphate (CMP) were the best inducers. Five mmol/L to fifteen mmol/L cytidine or CMP could distinctly increase the activities of purine nucleoside phosphorylase (PNPase), uridine phosphorylase (UPase) and thymidine phosphorylase (TPase) when they were added into medium from 0 to 8 h. In the process of enzymatic synthesis of adenine arabinoside from adenine and uracil arabinoside with wet cells of Enterobacter aerogenes DGO-04 induced by cytidine or CMP, the reaction time could be shortened from 36 to 6 h. After enzymatic reaction the activity of NPase in the cells induced remained higher than that in the cells uninduced.
