Recommendation of a highly sensitive method for measuring hemoglobin in hemolytic test.
- Author:
Wangping HU
1
;
Yingying HU
;
Fuying FENG
;
Jinyao HUANG
;
Rongsheng ZHANG
;
Rongren CHEN
;
Changshao ZHOU
;
Hailin WANG
Author Information
1. Clinical Chemistry Department,Fuzhou Genenal Hospital, Fuzhou 350025, China. HWP9999@sohu.com
- Publication Type:Journal Article
- MeSH:
Chlorophenols;
Coombs Test;
methods;
Hemoglobins;
analysis;
Hemolysis;
Humans;
Sensitivity and Specificity
- From:
Journal of Biomedical Engineering
2007;24(3):664-666
- CountryChina
- Language:Chinese
-
Abstract:
In this paper is recommended a highly sensitive and reagent-safe method to determine plasma heamoglobin (FHb) in viscacha hemolytic test. The 2,4-dichlorophenol method (2,4-DCP) of Trinder reaction has been improved. The performance of 2,4-DCP is verified. The sensitivity of 2,4-DCP is 2.39 times that of phenol method. It is well used with run precision and day-to-day precision. The reaction color is stable. The reference value FHb is 1-36.7 mg/L. Sodium citric is an excellent anticoagulant liquid to keep erythrocyte. The 2,4-DCP method is neither carcinogenic nor poisonous;it is suitable for viscacha hemolytic test in clinical and biomedical engineering.
