Influence on Indian hedgehog-parathyroid hormone-like related protein pathway induced by altered masticatory loading in the condylar cartilage of growing rabbits.
- VernacularTitle:不同咀嚼负荷对幼兔髁突软骨内印度豪猪蛋白-人甲状旁腺激素相关蛋白通路表达的影响
- Author:
Fan YAN
1
;
Jianying FENG
1
;
Chenyan LIU
2
Author Information
- Publication Type:Journal Article
- Keywords: Indian hedgehog; condylar cartilage; mastication; parathyroid hormone-like related protein
- MeSH: Animals; Blotting, Western; Cartilage; Chondrocytes; Hedgehog Proteins; Immunohistochemistry; Mastication; Parathyroid Hormone; Rabbits; Real-Time Polymerase Chain Reaction
- From: West China Journal of Stomatology 2017;35(2):127-132
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the influence of altered masticatory loading on Indian hedgehog (Ihh)-parathyroid hormone-like related protein (PThrP) pathway in the condylar cartilage of growing rabbits.
METHODSA total of 48 10-day-old rabbits were randomly divided into two groups and fed different kinds of food, such as solid diet and soft diet. The animals were sacrificed after 2, 4, 6, and 8 weeks. Difference of Ihh and PThrP expression levels induced by altered masticatory loading was tested by hematoxylin-eosin (HE), immunohistochemistry, Western blot, and real-time polymerase chain reaction (PCR).
RESULTSThe thickness of condylar cartilage and expression levels of Ihh and PThrP proteins and mRNA of the solid diet groups exceeded those of the soft diet groups. The decreasing tendencies of the expression levels of Ihh and PThrP proteins and mRNA were observed at 2, 4, 6, 8 weeks.
CONCLUSIONSLow masticatory loading may delay or inhibit the development of condylar cartilage and its growing factors Ihh and PThrP. Therefore, masticatory loading plays an important role in the development of condylar cartilage.
